Breast Aneusomy Multi-Color Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis® LSI® 1 | 1p12 | SpectrumGold TM |
| Vysis CEP® 8 | 8p11.1-q11.1 Alpha Satellite DNA | SpectrumRed TM |
| Vysis CEP 11 | 11p11.11-q11 Alpha Satellite DNA | SpectrumGreen TM |
| Vysis CEP 17 | 17p11.1-q11.1 Alpha Satellite DNA | SpectrumAqua TM |
Aneusomy in breast cancer has been well-documented and is considered an early event in breast carcinoma. In a study involving 106 fine needle aspirates of primary breast tumors, 96% of malignant tumors showed aneusomy of one or more of chromosome 1, 11, or 17, and no aneusomy in benign tumors.Another study found that chromosome 1, 11, and 17 were aneusomic in 79%, 59%, and 59% of 113 fine needle aspirates of breast tumors, respectively. Chromosome 8 was found to be the most frequent aneusomy out of 11 different chromosomes in a study of 55 breast tumor touch preparations.
For Research Use Only. Not for use in Diagnostic Procedures.
The Breast Aneusomy Probe was designed as a research tool to help study aneusomies and patterns of aneusomy that can provide important information as it pertains to prognosis, progression, recurrence, and metastasis. This probe has been optimized for use in cytology specimens.
DNA Probe Description
The LSI 1 probe is an approximately 470 kb clone contig positioned at 1p12. The probe is direct labeled in SpectrumGold.
The CEP 8 alpha satellite DNA probe hybridizes to the centromere region of chromosome 8. The probe is directly labeled with SpectrumRed (8p11.1-q11.1).
The CEP 11 alpha satellite DNA probe hybridizes to the centromere region of chromosome 11. The probe is directly labeled with SpectrumGreen (11p11.11-q11).
The CEP 17 alpha satellite DNA probe hybridizes to the centromere region of chromosome 17. The probe is directly labeled with SpectrumAqua (17p11.1-q11.1).
For Research Use Only. Not for use in Diagnostic Procedures.
The Breast Aneusomy Probe was designed as a research tool to help study aneusomies and patterns of aneusomy that can provide important information as it pertains to prognosis, progression, recurrence, and metastasis. This probe has been optimized for use in cytology specimens.
DNA Probe Description
The LSI 1 probe is an approximately 470 kb clone contig positioned at 1p12. The probe is direct labeled in SpectrumGold.
The CEP 8 alpha satellite DNA probe hybridizes to the centromere region of chromosome 8. The probe is directly labeled with SpectrumRed (8p11.1-q11.1).
The CEP 11 alpha satellite DNA probe hybridizes to the centromere region of chromosome 11. The probe is directly labeled with SpectrumGreen (11p11.11-q11).
The CEP 17 alpha satellite DNA probe hybridizes to the centromere region of chromosome 17. The probe is directly labeled with SpectrumAqua (17p11.1-q11.1).
Probe Map
A diagram of the 1p12 region and sequence tagged sites (STS) known to be contained within this region. The yellow bar represents the approximately ~470kb region covered by LSI 1 probe. Map not to scale.
Results of Hybridization
In a normal diploid cell, two copies of each of LSI 1 (gold signals), CEP 8 (red signals), CEP 11 (green signals), and CEP 17 (aqua signals) should be observed.
Normal results observed in an interphase cell obtained from a Fine Needle Aspirate sample after the Breast Aneusomy probe hybridization. Each probe signal: LSI 1 (gold), CEP 8 (red), CEP 11 (green), and CEP 17 (aqua) is present in 2 copies.
Aneusomic interphase cell obtained from a fine needle aspirate sample showing 3 copies of LSI 1(gold), 3 copies of CEP 8 (red), 3 copies of CEP 11 (green), and 4 copies of CEP 17 (aqua) after Breast Aneusomy probe hybridization.
For Research Use Only.
Not for use in diagnostic procedures.
Not for use in diagnostic procedures.
