Vysis® LSI® ALK Dual Color, Break Apart Rearrangement Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI ALK | 2p23 | SpectrumOrange TM SpectrumGreen TM |
The LSI ALK (Anaplastic Lymphoma Kinase) Dual Color, Break Apart Rearrangement Probe is designed to detect the known 2p23 rearrangements that occur in t(2;5) and its variants. The t(2;5) has been shown to fuse the nucleophosmin (NPM) gene located on chromosome 5q35 with the gene on chromosome 2p232. This NPM/ALK gene fusion gives rise to a chimeric protein that is overexpressed.The LSI ALK Dual Color, Break Apart Rearrangement Probe contains two differently labeled probes on opposite sides of the breakpoint of the ALK gene. An approximately 250 kb probe for the telomeric side of the ALK breakpoint is labeled with SpectrumOrange. The centromeric probe is approximately 300 kb and labeled with SpectrumGreen.
Probe Map
Results of Hybridization
When hybridized with the LSI ALK Dual Color, Break Apart Rearrangement Probe, the 2p23 ALK region in its native state will be seen as two immediately adjacent or fused orange/green (yellow) signals (2F). However, if a t(2;5) or other chromosome rearrangement at the 2p23 ALK breakpoint region has occurred, one orange and one green signal will be seen, while the native ALK region will remain as an orange/green fusion signal (1O1G1F). The hybridization result of the LSI ALK Dual Color, Break Apart Rearrangement Probe containing the t(2;5) translocation will be the centromeric green probe remaining at 2p23, while the telomeric orange signal that covers the region is translocated to 5q35 on the derivative chromosome.
LSI ALK Dual Color, Break Apart Rearrangement Probe hybridized to nuclei lacking the ALK gene rearrangement showing a two orange/green fusion (2F) signal pattern.
Analyte Specific Reagent.
Analytical and performance characteristics are not established.
Analytical and performance characteristics are not established.
