Vysis® LSI® IGH Dual Color, Break Apart Rearrangement Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI IGH | 14q32 | SpectrumOrange TM SpectrumGreen TM |
The LSI IGH Dual Color, Break Apart Rearrangement Probe is designed to detect chromosomal breakage of the immunoglobulin heavy chain (IGH) locus that is associated with 14q32 translocations involving a variety of other loci. Breakpoints within the IGH locus may occur at either the J segments [e.g., breakpoints commonly observed with t(14;18)] or within switch sequences located within the constant gene segments.The LSI IGH Dual Color, Break Apart Rearrangement Probe is a mixture of two probes that hybridize to opposite sides of the J through constant regions of the IGH locus. The approximately 900 kb SpectrumGreen labeled LSI IGHV probe covers essentially the entire IGH variable region. The hybridization target of the approximately 250 kb SpectrumOrange labeled LSI IGH 3' flanking probe lies completely 3' to the IGH locus. As a result of this probe design, any translocation with a breakpoint at the J segments or within switch sequences should produce separate orange and green signals.
Probe Map
Results of Hybridization
When hybridized to a normal nucleus, the LSI IGH Dual Color, Break Apart Rearrangement Probe produces a two orange/green (yellow) fusion (2F) signal pattern. As there is no probe targeted to the J or constant regions, a slight gap between the two differently colored probe signals may sometimes be observed in nuclei from normal cells. When the IGH Dual Color, Break Apart Translocation Probe is hybridized to a nucleus containing an IGH translocation, one orange, one green, and one orange/green fusion signal pattern is observed (1O1G1F). This signal pattern indicates that the genomic targets for the LSI IGHV and LSI IGH 3' flanking probes have been physically separated as a result of the translocation. As V(D)J rearrangements may occur on either, or both, of the translocated and non-translocated IGH alleles, the green LSI IGHV probe signal intensity on either, or both, of the alleles may be diminished as a result of probe target deletion in some samples.
LSI IGH Dual Color, Break Apart Rearrangement Probe hybridized to nuclei exhibiting the expected two fusion (2F) signal pattern.
Analyte Specific Reagent.
Analytical and performance characteristics are not established.
Analytical and performance characteristics are not established.
