Vysis® LSI® p53 / LSI ATM and LSI D13S319 / LSI 13q34 / CEP® 12 Multi-color Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI p53 | 17p13.1 | SpectrumOrange TM |
| Vysis LSI ATM | 11q22.3 | SpectrumGreen TM |
| Vysis LSI S13S319 | 13q14.3 | SpectrumOrange TM |
| Vysis LSI 13q34 | 13q34 | SpectrumAqua TM |
| Vysis CEP 12 | 12p11.1-q11 | SpectrumGreen TM |
Probe vial 1 contains LSI p53 in SpectrumOrange and LSI ATM in SpectrumGreen. Probe vial 2 contains LSI D13S319 in SpectrumOrange, LSI 13q34 in SpectrumAqua, and CEP 12 in SpectrumGreen.
The LSI p53 (17p13.1) probe is a ~145 kb unique sequence probe that is labeled in SpectrumOrange.
The LSI ATM probe is a ~500 kb unique sequence probe that hybridizes to the 11q22.3 region of chromosome 11. This probe spans the entire ~184 kb Ataxia telangiectasia mutated (ATM) gene and several others. The probe is labeled in SpectrumGreen.
The LSI D13S319 probe is a ~130 kb unique sequence probe that is labeled in SpectrumOrange.
The LSI 13q34 probe is a ~550 kb unique sequence probe that hybridizes to the 13q34 region containing the Lysosomal-associate Membrane Protein (LAMP1) gene and several others. The probe is labeled in SpectrumAqua.
The CEP 12 DNA probe hybridizes to the alpha satellite (centromeric) region (12p11.1-q11) of chromosome 12. The probe is labeled in SpectrumGreen.
The LSI p53 (17p13.1) probe is a ~145 kb unique sequence probe that is labeled in SpectrumOrange.
The LSI ATM probe is a ~500 kb unique sequence probe that hybridizes to the 11q22.3 region of chromosome 11. This probe spans the entire ~184 kb Ataxia telangiectasia mutated (ATM) gene and several others. The probe is labeled in SpectrumGreen.
The LSI D13S319 probe is a ~130 kb unique sequence probe that is labeled in SpectrumOrange.
The LSI 13q34 probe is a ~550 kb unique sequence probe that hybridizes to the 13q34 region containing the Lysosomal-associate Membrane Protein (LAMP1) gene and several others. The probe is labeled in SpectrumAqua.
The CEP 12 DNA probe hybridizes to the alpha satellite (centromeric) region (12p11.1-q11) of chromosome 12. The probe is labeled in SpectrumGreen.
Probe Map
Results of Hybridization
This multi-color probe set is provided in a two-mixture format. A description of the hybridization results expected with each mixture follows:Probe 1:
This probe allows status assessment of the following two chromosome regions: 17p13.1 (p53) and 11q22.3 (ATM). In a normal cell with two intact copies of chromosomes 17 and 11, two orange, and two green signals will be observed.
Probe 2:
This probe allows status assessment of the following three chromosome regions:
13q14.3 (D13S319), 13q34, and 12p11.1-q11. In a normal cell with two intact copies of chromosome 13 and chromosome 12, a two orange, two aqua, and two green signal pattern will be observed.
An abnormal cell hybridized with Probe Set 1.
An abnormal cell hybridized with Probe Set 1.
An abnormal cell hybridized with Probe Set 2.
An abnormal cell hybridized with Probe Set 2.
Analyte Specific Reagent.
Analytical and performance characteristics are not established.
Analytical and performance characteristics are not established.
