HSV-1/2 PCR Kit

The HSV-1/2 PCR Kit constitutes a ready-to-use system for the detection and differentiation of herpes simplex virus-1 and -2 DNA using polymerase chain reaction (PCR) in the ABI PRISM® 7000 and 7900HT Sequence Detection System (Applied Biosystems). The HSV TM Master contains reagents and enzymes for the specific amplification of a 148 bp region of the herpes simplex virus genome. The amplicon is detected by measuring the FAM fluorescence (HSV-1) and NED fluorescence (HSV-2) in the ABI PRISM® SDS. In addition, the HSV-1/2 PCR Kit contains a second heterologous amplification system to identify possible PCR inhibition. This is detected as an Internal Control (IC) by measuring the VIC fluorescence. External positive controls (HSV1 LC/RG/TM QS 1 - 4 & HSV2 LC/RG/TM QS 1 - 4) are supplied which allow the determination of the pathogen load.