M. tuberculosis PCR Kit

The M. tuberculosis PCR Kit constitutes a ready-to-use system for the detection of DNA of all members of the M. tuberculosis complex (M. tuberculosis, M. africanum, M. bovis, M. bovis BCG, M. microti, M. pinnipedii) using polymerase chain reaction (PCR) in the ABI PRISM® 7000, 7700 and 7900HT Sequence Detection System (Applied Biosystems). The M. tuberculosis TM Master contains reagents and enzymes for the specific amplification of a 159 bp region of the mycobacterial genome. The amplicon is detected by measuring the FAM fluorescence in the ABI PRISM® SDS. In addition, the M. tuberculosis PCR Kit contains a second heterologous amplification system to identify possible PCR inhibition. This is detected as an Internal Control (IC) by measuring the JOE fluorescence.  External positive controls (M. tuberculosis RG/TM QS 1 - 4) are supplied which allow the determination of the pathogen load.