SSP

SSP is a DNA genotyping approach based on the use of sequence-specific primers (SSP) in which a terminal 3’single base mismatch inhibits the priming of the non-specific DNA templates. Due to a lack of 3’ to 5’ exonuclease proof-reading activity by Taq-polymerase, only completely 3’- matched templates will be efficiently amplified. This approach is used to amplify specific HLA alleles or allele groups. When the corresponding alleles are present, their specific amplicons can be visualized by gel electrophoresis.

SSP tests are currently used for rapid genotyping, confirmation typing and screening for single alleles.

• SSP test consists of multiple different PCR primers specific for known HLA polymorphisms
• Multiple PCRs are prepared for each sample and the specific amplicons will be produced if the primers are complementary to the sample DNA
• Process requires only a thermal cycler and basic gel electrophoresis equipment