Required Vysis Filter Set Configurations
The following filter set configurations are required for the specific Vysis Assays, as indicated. The recommended filter sets provide the most optimal viewing conditions. If not indicated, contact your local Abbott Molecular Technical Service representative for more information on appropriate filter set configurations for imaging.
PathVysion HER-2 DNA Assay Filter Sets
The following filter sets are recommended for viewing and enumeration of the PathVysion HER-2 Assay. These filter sets are optimized both for Vysis SpectrumGreen and SpectrumOrange fluorophores and for paraffin-embedded specimen autofluorescence. The dual bandpass filter sets allow the user to view signals of each respective individual color fluorophore and the DAPI counterstain. The triple bandpass filter set allows the user to visualize the SpectrumGreen, SpectrumOrange, and DAPI fluorescent signals simultaneously.
| Vysis Filter Set |
Fluorophores Detected |
| DAPI/9-Orange (NB) dual bandpass | SpectrumOrange and DAPI |
| DAPI/Green dual bandpass | SpectrumGreen and DAPI |
| DAPI/Green/Orange (V.2) triple bandpass | SpectrumGreen/SpectrumOrange/DAPI (specifically designed for viewing paraffin sections) |
AneuVysion Assay Filter Sets
The following filter set configuration provides the best microscope filter set-up for viewing and analysis of the AneuVysion Assay on uncultured amniocytes.
| Vysis Filter Set |
Fluorophores Detected |
| Aqua single bandpass |
SpectrumAqua |
| Green single bandpass | SpectrumGreen |
| Orange single bandpass | SpectrumOrange |
| DAPI/Green/Orange triple bandpass |
SpectrumGreen/SpectrumOrange/ DAPI |
UroVysion Assay Filter Sets
| Vysis Filter Set |
Fluorophores Detected |
| DAPI single bandpass |
DAPI |
| Aqua single bandpass | SpectrumAqua |
| Yellow single bandpass | SpectrumGold |
| Red/Green dual bandpass | SpectrumRed/SpectrumGreen |
