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CGH Reagents

For Laboratory Use
View Ordering Information
Product Name Quantity Order # Additional Information
CGH Metaphase Target Slides 10 Slides 06J96-001 Order
Control DNA Unlabeled 15 µL 06J40-001 Order
SpectrumGreen Control DNA 25 µL 06J45-001 Order
CGH Nick Translation Kit With Control DNA (MPE 600) 50 Reactions 06J40-020 Order
SpectrumGreen Normal Female Reference DNA 300 ng/µL x 25 uL 07J03-001 Order
SpectrumGreen Normal Male Reference DNA 300 ng/µL x 25 uL 07J03-005 Order
SpectrumRed Normal Female Reference DNA 100 ng/µL x 25 µL 07J04-001 Order
SpectrumRed Normal Male Reference DNA 100 ng/µL x 25 µL 07J04-005 Order
CGH Hybridization Reagents 50 Reactions 06J40-010 Order
Human COT-1 DNA 250 µL 06J31-001 Order

CGH Metaphase Target Slides

Metaphase slides prepared from normal PHA-stimulated peripheral blood lymphocytes for optimum in situ hybridization results in CGH.

(10 slides = 20 hybridization areas)

Control DNA Unlabeled

This control DNA is extracted from the MPE-600 tumor cell line that has known chromosome aberrations determined from CGH and karyotype analysis. Expected CGH results for the control DNA are described to provide control data for evaluation of hybridization efficiency of CGH techniques.

SpectrumGreen Control DNA

This product is provided as positive control DNA for CGH. The DNA is extracted from the MPE-600 tumor cell line that has known chromosome aberrations determined from CGH and karyotype analysis. The DNA is directly labeled with SpectrumGreen fluorophore.

Controls may be useful as comparison for fluorophore labeling and hybridization results.

This control is also provided in the CGH Nick Translation Kit.

CGH Nick Translation Kit With Control DNA (MPE 600)

The CGH Nick Translation Kit is used to label DNA (that is isolated for CGH) with either SpectrumGreen or SpectrumRed™ fluorophore.

Components in the CGH Nick Translation Kit include:

  • Two positive controls
    • Unlabeled DNA (3 µg) extracted from the MPE-600 cell line
    • SpectrumGreen labeled MPE-600 DNA (1.5 µg)
    • Nick translation enzyme mix (525 µL)
  • 10X Nick translation buffer (300 µL)
  • dATP, dCTP, dGTP, and dTTP solutions (200 µL each)
  • Nuclease-free water (1500 µL)
  • Easy-to-follow protocol

Storage Conditions: -20°C

CGH Hybridization Reagents

CGH Hybridization Reagents are used to prepare a CGH hybridization following probe preparation.

Hybridization Reagents include:

  • CGH Hybridization Buffer, 250 ìL
  • 20X SSC, 66 g
  • NP-40, 1000 ìL
  • DAPI II Counterstain with antifade, 250 ìL
  • CGH protocol

Human COT-1 DNA

COT-1 blocking DNA for use in FISH/CGH.

Storage Conditions: -20°C.

SpectrumGreen Normal Female Reference DNA

Labeled Reference DNA:
Ready-to-use fluorophore-labeled female total human genomic DNA is isolated from normal lymphocytes for use in CGH. The reagent does not contain COT-1® DNA. The DNA was extracted from the peripheral blood of karyotypically normal female individuals and contains minimal polymorphic regions.

SpectrumGreen Normal Male Reference DNA

Labeled Reference DNA:
Ready-to-use fluorophore-labeled male total human genomic DNA is isolated from normal lymphocytes for use in CGH. The reagent does not contain COT-1 DNA. The DNA was extracted from the peripheral blood of karyotypically normal male individuals and contains minimal polymorphic regions.

SpectrumRed Normal Female Reference DNA

Labeled Reference DNA:
Ready-to-use fluorophore-labeled female total human genomic DNA is isolated from normal lymphocytes for use in CGH. The reagent does not contain COT-1 DNA. The DNA was extracted from the peripheral blood of karyotypically normal female individuals and contains minimal polymorphic regions.

SpectrumRed Normal Male Reference DNA

Labeled Reference DNA:
Ready-to-use fluorophore-labeled male total human genomic DNA is isolated from normal lymphocytes for use in CGH. The reagent does not contain COT-1 DNA. The DNA was extracted from the peripheral blood of karyotypically normal male individuals and contains minimal polymorphic regions.

For Laboratory use.