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Hybridization Reagents

For Laboratory Use
View Ordering Information
Product Name Quantity Order # Additional Information
NP-40 1000 µL x 2 07J05-001 Order
20X SSC 500 g 02J10-032 Order
LSI/WCP® Hybridization Buffer 2 x 500 µL 06J67-011 Order
LSI/WCP® Hybridization Buffer 2 x 150 µL 06J67-001 Order
CEP Hybridization Buffer 2 x 150 µL 07J36-001 Order
Human COT-1 DNA 250 µL 06J31-001 Order
Antifade Solution 240 µL x 2 06J29-010 Order
Antifade II Solution 60 µL x 2 06J29-001 Order
Propidium Iodide Counterstain 500 µL x 2 07J06-001 Order
DAPI I Counterstain 500 µL x 2 06J49-001 Order
DAPI II Counterstain 500 µL x 2 06J50-001 Order
DAPI III Counterstain 500 µL x 2 06J49-010 Order
Nick Translation Kit 50 Reactions 07J00-001 Order

NP-40

NP-40 is Nonidet P-40 and is a non-ionic detergent used in the aqueous post-hybridization wash solution and is necessary for product performance.

20X SSC

20X SSC is a powder containing sodium chloride and sodium citrate. It is used to make 20X SSC solution and subsequent dilutions. (2X SSC and 0.4X SSC) for denaturization and wash solutions.

LSI/WCP Hybridization Buffer

LSI/WCP Hybridization Buffer is optimized for performance with LSI and WCP reagents. This product contains dextran sulphate, formamide and SSC (pH=7.0).

  • Certificate of Analysis highlights quality control data and key product information

CEP Hybridization Buffer

CEP Hybridization Buffer is optimized for performance with CEP probes and contains dextran sulfate, formamide, and SSC (pH 7.0).

Human COT-1 DNA

COT-1 blocking DNA for use in FISH/CGH. Storage Conditions: -20°C.

Antifade Solution

Antifade Solution is comprised of 1,4-phenylenediamine and glycerol in an aqueous buffer solution.

Antifade II Solution

Antifade II Solution is a special formulation of antifade and mounting buffer.

Propidium Iodide Counterstain

May be used as a counterstain for SpectrumGreen™ or SpectrumAqua™ CEP probes.

DAPI I Counterstain

Recommended counterstain when a more intense DAPI stain is desired. Provides more distinct DAPI banding patterns than achievable with DAPI II in certain applications such as staining of nuclear DNA in paraffin sections.

DAPI II Counterstain

DAPI II is the recommended counterstain for most CEP and LSI probes. This concentration of DAPI provides a weaker DAPI stain usually preferred with smaller probe signals such as CEP and LSI probes.

DAPI III Counterstain

DAPI III is a dilute counterstain that is optimized for DAPI banding of chromosomes used in fluorescence imaging. DAPI III may also be optimal as a counterstain for imaging applications using other CEP, and LSI probes.

Nick Translation Kit

The Nick Translation Kit is designed for fluorescence labelling of DNA using Vysis fluorophore-labeled dUTPs.

For Laboratory use.